Regulatory
Part:BBa_K1592022:Design
Designed by: Shuyan Tang Group: iGEM15_HUST-China (2015-09-09)
ptrp mutant3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
As the trp promoter has two illegal restriction enzyme sites (SpeI),we designed the point mutantion to improve it.
Source
Cloned from BBa_K588001 and mutated by overlap extension PCR.